In separation-based land use (zoning) theory, due to the contrasting nature of different land use categories to each other, a separation among residential areas and other land uses were assumed. During recent decades and after revealing negative effects of zoning, new urbanism approaches based on mixed land use development and revival of urban district and neighbourhood centres have been proposed. The aim of this research is locating and ranking urban district and neighbourhood centres based on examination of mixed land use index in GIS environment. In this article, a GIS-based model is developed through measurement of five spatial indices. In order to‏ evaluate the efficiency of the proposed model, it was applied in the seventh region of Tehran with its five neighbourhoods. Evaluation of the results was demonstrated the appropriate performance of the model in detecting network nodes which have good potential as district or neighbourhood centres in the study area. These results have good reliability with the comprehensive plan of the case study.